Authors: Reeta Davis, Gearoid Duane, Shane T Kenny, Federico Cerrone, Maciej W Guzik, Ramesh P Babu, Eoin Casey, Kevin E O’Connor
Publication Date: 04/2015
Journal: Biotechnology and Bioengineering
Volume: 112
Issue: 4
Pages: 725-733
High Cell Density (HCD) cultivation of bacteria is essential for the majority of industrial processes to achieve high volumetric productivity (g L−1 h−1) of a bioproduct of interest. This study developed a fed batch bioprocess using glucose as sole carbon and energy source for the HCD of the well described biocatalyst Pseudomonas putida KT2440 without the supply of oxygen enriched air. Growth kinetics data from batch fermentations were used for building a bioprocess model and designing feeding strategies. An exponential followed by linearly increasing feeding strategy of glucose was found to be effective in maintaining biomass productivity while also delaying the onset of dissolved oxygen (supplied via compressed air) limitation. A final cell dry weight (CDW) of 102 g L−1 was achieved in 33 h with a biomass productivity of 3.1 g L−1 h−1 which are the highest ever reported values for P. putida strains using …
